Galaxy NUBRI: Democratizing Bioinformatics in Sudan
In Silico
Galaxy NUBRI: Democratizing Bioinformatics in Sudan
Overview
Galaxy NUBRI enables users with minimal programming experience to perform complex bioinformatics analyses via a simple web interface. The platform supports a wide range of applications, including next-generation sequencing (NGS) workflows, variant calling, RNA-Seq analysis, metagenomics, epitope prediction, and more.
Objectives
- To install and deploy a local Galaxy instance at NUBRI with essential bioinformatics tools and workflows.
- To support genomic research, diagnostics, and drug discovery through an intuitive platform accessible to wet-lab scientists and clinicians.
- To train staff, students, and external collaborators in the use of Galaxy for NGS data analysis, AMR surveillance, cancer genomics, and more.
- To establish long-term collaboration with the African Galaxy Project and enable remote job execution and tool sharing with regional partners.
- To ensure sustainability by building local technical capacity in server management, tool integration, and pipeline development.
- National University – Sudan
Research Phases
Phase One: Local Deployment of the Galaxy Platform
The steps successfully completed in Phase One included:
- The server environment was configured using a Linux-based infrastructure, with all necessary dependencies and core Galaxy tools fully integrated.
- A dedicated local web interface was established, enabling researchers at NUBRI and affiliated institutions to access bioinformatics workflows and pipelines related to genomics, transcriptomics, and molecular diagnostics.
- Quality control of sequencing data (e.g., FastQC, Trimmomatic)
- Genome assembly and annotation
- Variant calling and alignment (e.g., BWA, GATK)
- Gene expression analysis and visualization
- Custom workflows for antimicrobial resistance and cancer genomics
Phase Two: Regional Integration with African Galaxy Network (Suspended)
The second phase aimed to integrate the local NUBRI Galaxy server with the African Galaxy Platform, a federated bioinformatics initiative coordinated by SANBI.
- Enable cross-border data sharing and workflow harmonization among African research institutions
- Provide access to cloud-based high-performance computing (HPC) resources
- Facilitate collaborative training programs and tool development through regional partnerships
Future Plans
Upcoming developments to enhance Galaxy NUBRI functionality and collaboration:
- Upgrade the Galaxy server with new tools for multi-omics integration and machine learning applications
- Resume collaboration with SANBI for platform federation and remote job execution
- Offer structured Galaxy training workshops for students and researchers in Sudan and the broader region